Epstein Barr virus VCA IgM ELISA Kit

Short Description:

It is used for qualitative detection of IgM-class antibodies to Epstein-barr virus capsid antigen in human serum or plasma. It is intended to be used in clinical laboratories for diagnosis and management of patients related to infection with Epstein-barr virus.

EBV infection is widespread, with more than 90% of the world’s population having been infected with the virus, and EBV is characterized as an insidious infection, with primary infections occurring in infants and adolescents, mainly as asymptomatic, and adolescents presenting with infectious mononucleosis. After primary infection, EBV is usually latent in human mature B lymphocytes. Under certain conditions, the latent virus can be activated, stimulating cell proliferation and differentiation, and in some cases, eventually transforming into malignant diseases such as lymphoma, with a poor prognosis, so early detection of EBV should not be ignored.

EBV is one of the causes of several malignancies (e.g. nasopharyngeal carcinoma) and it mainly infects epithelial cells and B lymphocytes in the human oropharynx. EBV tests have antibody and antigen tests. EBV antibody tests include antibodies related to viral capsid antigen (VCA), early antigen (EA), viral nuclear antigen (EBNA), and membrane antigen (MA), and are commonly used clinically to detect EB-VCA-IgM and EB-VCA-IgG. The EB-VCA-IgM test detects antibodies in the acute phase of the patient, and a positive result for this item is Early, specific and sensitive basis for clinical diagnosis.

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Product Detail

Product Tags

Principle

This kit uses the principle of indirect method to detect EBVCA IgM antibody serum or plasma samples, polystyrene microwell strips are pre-coated with antibodies directed to human immunoglobulin M proteins (anti-μ chain).After first adding serum or plasma specimens to be examined, the IgM antibodies in the specimen can be captured, and other unbound components (including specific IgG antibodies) will be removed by washing. In the second step, the HRP (horseradish peroxidase)-conjugated antigens will specifically react only with EBV IgM antibodies. Finally, the TMB substrate was added for color development. The presence of the absorbance (A value) of the EBVCA IgM antibody in the sample was determined by a microplate reader.

Product Features

High sensitivity, specificity and stability

Product Specification

Principle	Enzyme linked immunosorbent assay
Type	Capture method
Certificate	CE
Specimen	Human serum / plasma
Specification	48T / 96T
Storage temperature	2-8℃
Shelf life	12 months