Hepatitis D virus IgM ELISA Kit

Short Description:

Hepatitis D virus IgM ELISA Kit is an enzyme-linked immunosorbent assay for the qualitative detection of IgM-class antibodies to hepatitis D virus in human serum or plasma. It is intended to be used in clinical laboratories for diagnosis and management of patients related to infection with hepatitis D virus. A positive anti-HDV-IgM indicates an acute or recent HDV infection; a co-infection with HDV/HBV is associated with a transient positive anti-HDV-IgM; an overlapping infection is associated with a persistent positive anti-HDV-IgM; therefore, the anti-HDV-IgM test has an important diagnostic value. Anti-HDV-IgM antibodies can distinguish between recent or previous infections, co-infections and overlapping infections, and persistent high titers of anti-HDV-IgG and IgM fluctuate repeatedly, which are important indicators of chronic HDV infections, so detection of anti-HDV-IgM is important for the diagnosis and prognosis of HDV infections.


Product Detail

Product Tags

Principle

This kit detects hepatitis D virus IgM antibody (HDV-IgM) in human serum or plasma samples, polystyrene microwell strips are pre-coated with antibodies directed to human immunoglobulin M proteins (anti-μ chain). After first adding serum or plasma specimens to be examined, the IgM antibodies in the specimen can be captured, and other unbound components (including specific IgG antibodies) will be removed by washing. In the second step, the HRP (horseradish peroxidase)-conjugated antigens will specifically react only with HDV IgM antibodies. After washing to remove the unbound HRP-conjugate, chromogen solutions are added into the wells. In presence of (anti-μ) - (HDV-IgM) - (HDV Ag-HRP) immunocomplex, after washing the plate, the TMB substrate was added for color development, and the HRP connected to the complex catalyzes the color developer reaction to generate the blue substance, add 50μl of Stop Solution, and turn yellow. The presence of the absorbance of the HDV-IgM antibody in the sample was determined by a microplate reader.

Product Features

High sensitivity, specificity and stability

Product Specification

Principle Enzyme linked immunosorbent assay
Type Capture method
Certificate NMPA
Specimen Human serum / plasma
Specification 48T / 96T
Storage temperature 2-8℃
Shelf life 12 months

Ordering Information

Product name Pack Specimen
Hepatitis D virus IgM ELISA Kit 48T / 96T Human serum / plasma

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