SARS-COV-2 Total Ab Test Kit (Elisa)

Short Description:

The SARS-CoV-2 Total Ab Test Kit (ELISA) is an enzyme-linked immunosorbent assay for qualitative detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgG/IgM antibody in human serum, plasma (EDTA, Heparin or sodium citrate) sample. The test is to be used as an aid in the diagnosis of coronavirus infection disease (COVID-19), which is caused by SARS-CoV-2.


Product Detail

Product Tags

Principle

The SARS-CoV-2 Total Ab Test Kit (ELISA) is based on immunoenzymatic assay for determination of SARS-CoV-2 antibodies in human serum, plasma (EDTA, Heparin or sodium citrate). The micro plate wells as a solid phase are coated with recombinant SARS-CoV-2 recombinant Receptor Binding Domain protein. In the first incubation step corresponding specific antibodies (SARS-CoV-2-IgG-Ab & some IgM-Ab) present in patient specimens bind to the antigens at the solid phase. At the end of the incubation unbound components are washed out. For the second incubation step SARS-CoV-2 recombinant Receptor Binding Domain protein conjugate (SARS-CoV-2 recombinant Receptor Binding Domain protein peroxidase conjugate) is added which binds specifically to SARS-CoV-2 antibodies (including IgG and IgM) resulting in the formation of typical immunocomplexes. After a second washing step to remove excess conjugate, TMB/Substrate is added (step3). A blue color develops changing to yellow after stopping the reaction with stop solution. The absorbance of calibrators and specimen is determined by using an ELISA micro plate reader. Results for patient samples are obtained by comparison with a cut-off value.

Product Features

High sensitivity, specificity and stability

Product Specification

Principle Enzyme linked immunosorbent assay
Type Sandwich Method
Certificate CE
Specimen Human serum / plasma
Specification 96T
Storage temperature 2-8℃
Shelf life 12 months

Ordering Information

Product name Pack Specimen
SARS-COV-2 Total Ab Test Kit (Elisa) 96T Human serum / plasma

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