Toxoplasma IgG ELISA Kit

This kit detects Toxoplasma lgG antibody (TOX-lgG) in human serum or plasma samples, polystyrene microwell strips are pre-coated with Toxoplasma antigen. After first adding serum or plasma specimens to be examined, corresponding specific antibodies (TOX-lgG-Ab & some lgM-Ab) present in patient specimens bind to the antigens at the solid phase, and other unbound components will be removed by washing. In the second step, the HRP(horseradish peroxidase)-conjugated anti-human lgG will specifically react only with TOX lgG antibodies. After washing to remove the unbound HRP-conjugate , chromogen solutions are added into the wells. In presence of (TOX Ag) - (TOX-lgG) - (anti-human lgG-HRP) immunocomplex, after washing the plate, the TMB substrate was added for color development, and the HRP connected to the complex catalyzes the color developer reaction to generate the blue substance, add 50 µ I of Stop Solution, and turn yellow. The presence of the absorbance of the TOX-lgG antibody in the sample was determined by a microplate reader.